Cloning of variable region and signal peptide genes of anti - cd20 monoclonal antibody by rlm - race 20單克隆抗體可變區(qū)基因及其信號(hào)肽基因
This paper studied the cloning signal peptide and promoter of grb - ast gene 本論文對(duì)grb - ast基因的信號(hào)肽序列及其啟動(dòng)子進(jìn)行分離克隆的研究。
In addition , the function of secreting protein by grb - ast signal peptide was also studied in this paper 此外,還探討了grb - ast信號(hào)肽分泌蛋白的功能。
Computer - assisted analysis revealed there was a signal peptide near the n - terminal of this protein 軟件預(yù)測(cè)0rf132有一個(gè)信號(hào)肽序列( signalpeptide ) ,最可能的切割位點(diǎn)在和r20之間。
Sequence analysis shows that the n - terrninal 1 - 25 amino acid sequence is a predicted signal peptide 序列分析也顯示蛋白n ?端具有信號(hào)肽序列特征,表明ecbp21可能是一種分泌到細(xì)胞外的蛋白。
Our data indicate that in the insect cells , we can express the human full - length gene carrying the introns and signal peptide 我們的結(jié)果表明在昆蟲(chóng)細(xì)胞中能夠正確的表達(dá)帶有自身內(nèi)含子和信號(hào)肽的人源蛋白基因。
The main results are the following : 1 . the hplap without signal peptide was expressed in p . partoris smd1 168 by the ppicz - a a plasmid . 2 在畢赤酵母幾psrtorjssmdi168e株中用表達(dá)載體ppiczqa成功表達(dá)了去掉信號(hào)肽的有活性的堿性磷酸酯酶hplap 。
Hydrophobicity analysis suggested that the protein was highly hydrophilic , especialy at the first 24 amino - acid , this region could be function as signal peptide 將此基因重組到表達(dá)質(zhì)粒載體pgex - 4t - 1上,轉(zhuǎn)化到大腸桿菌bl21 ( de3 )中,經(jīng)iptg誘導(dǎo)后,可得到高效表達(dá)。
In this paper , signal peptide was added before the n - terminus of cema with the intention to reduce its toxic to plant cells while maintaining the strong antibacterial activity 本文通過(guò)在cema的n端添加信號(hào)肽,對(duì)其進(jìn)行改造,以探索不降低抑菌作用的同時(shí),降低其對(duì)植物細(xì)胞毒害作用的可能性。
In this thesis , signal peptide analyse software and trans - menbrane helix analyse software were integrated in the research of mycobacterium tuberculosis ( mtb ) secreted proteins prediction 本研究嘗試了利用信號(hào)肽預(yù)測(cè)軟件和跨膜螺旋預(yù)測(cè)軟件對(duì)結(jié)核桿菌全蛋白組進(jìn)行分泌性蛋白的預(yù)測(cè)分析研究。
A signal peptide (sometimes referred to as signal sequence, leader sequence or leader peptide) is a short (5-30 amino acids long) peptide present at the N-terminus of the majority of newly synthesized proteins that are destined towards the secretory pathway.